What is a pipette tip and the function of a filter tip?
The pipette tip is supplied by the biological company: elisa animal serum, fluorescent quantitative PCR consumables, pipette nozzle, microcentrifuge tube, imported cryotube, cell culture dish, culture plate, culture bottle, imported tip, instrument and Gloves, chromatography consumables, syringe filters, etc.
The pipette is an experimental instrument of du* in biological research, and the number of its accessory suction heads in the experiment is also very large. The suction tips on the market are basically made of polypropylene plastic (colorless and transparent plastic with high chemical inertness and a wide temperature range). However, the quality of the same polypropylene will vary greatly: high-quality tips are generally made of natural polypropylene, while cheap tips are likely to use recycled polypropylene plastic (in this case, we can Said its main component is polypropylene).
In addition, most of the tips will add a small amount of additives during the manufacturing process, the common ones are:
1. Chromogenic material.
Commonly known in the market as blue tip (1000ul) and yellow tip (200ul), the corresponding color-developing material is added to polypropylene (we hope it is high-quality masterbatch, not cheap industrial pigments)
2. Release agent.
Help the tip to be separated from the mold quickly after forming. Of course, the more additives, the higher the probability of undesirable chemical reactions during pipetting. So fortunately, no additives are added! However, due to the relatively high requirements for the production process, nozzles that do not add additives at all are rare in the market.
The role of the tip filter:
Because the tip filter element is a secondary filter tip, its major function during use is to prevent cross-contamination: unlike other filter types that contain additives that can inhibit enzymatic reactions, the filtered pipette tips supplied by Bunsen are made of Made of pure virgin sintered polyethylene. Hydrophobic polyethylene particles prevent aerosols and liquids from being sucked into the pipette body.
The filter of the shampoo cartridge is loaded by the machine to ensure that it is completely unaffected during the manufacturing and packaging process. They are certified to be free of RNase, DNase, DNA and pyrogen contamination. In addition, all filters are pre-sterilized by radiation after packaging to enhance the protection of biological samples.
The use of filter tips can be used to prevent the pipette from being damaged by the sample and greatly increase the service life of the pipette.
When to use the tip filter:
When to use the tip filter tip? Filtered pipette tips must be used in all molecular biology applications that are sensitive to contamination. The filter tip helps reduce the possibility of smoke formation, prevents aerosol contamination, and thus protects the pipette shaft from cross-contamination. In addition, the filter barrier prevents the sample from being carried away from the pipette, thereby preventing PCR contamination.
The filter tip also prevents the sample from entering the pipette and causing damage to the pipette during pipetting.
Why do you have to use a tip filter to detect the virus?
The virus is contagious. If the filter tip is not used to isolate the virus in the sample during the virus detection process, it will cause the virus to be transmitted through the pipette;
The test samples are different, and the filter tip can organize the cross-contamination of the sample during the pipetting process.
