The basic principle and characteristics of nucleic acid extractor

Nucleic Acid Extraction System (Nucleic Acid Extraction System) is an instrument that uses matching nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. Widely used in disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, animal husbandry and molecular biology research and other fields.
1. The suction method, also known as the pipetting method, is to extract nucleic acid by immobilizing magnetic beads and transferring liquid. Generally, the transfer is realized by controlling the robotic arm through the operating system. The extraction process is as follows:

1) Lysis: Add lysis solution to the sample, and realize the mixing and full reaction of the reaction solution through mechanical movement and heating, the cells are lysed, and the nucleic acid is released.

2) Adsorption: Add magnetic beads to the sample lysate, mix thoroughly, and use the magnetic beads to have a strong affinity for nucleic acids under high salt and low pH to adsorb nucleic acids. Under the action of an external magnetic field, the magnetic beads are separated from the solution. , use the tip to remove the liquid and discard it to the waste tank, and discard the tip.

3) Washing: Remove the external magnetic field, replace with a new tip and add washing buffer, mix well to remove impurities, and remove the liquid under the action of the external magnetic field.

4) Elution: Remove the external magnetic field, replace with a new tip, add elution buffer, mix well, and then separate the bound nucleic acid from the magnetic beads to obtain purified nucleic acid.
2. Magnetic bar method

The magnetic rod method realizes the separation of nucleic acids by fixing the liquid and transferring the magnetic beads. The principle and process are the same as those of the suction method, but the difference is the separation method between the magnetic beads and the liquid. The magnetic bar method is to separate the magnetic beads from the waste liquid through the adsorption of the magnetic rod to the magnetic beads, and put them into the next liquid to realize the extraction of nucleic acid.